Using state of the art technology we can identify the site of expression of your gene of interest. Our service covers not only the in situ hybridisation experiment, but also all related technologies:
- Probe design and preparation - if you are having problems designing, preparing or labelling your own probes, let Orion perform these experiments for you.
- Protocol optimisation - a major problem with in situ hybridisation is optimising the experimental protocol - Orion has a vast experience of all types of probes and tissues so let us help to design your protocol, thus saving on time and money
- Full in situ hybridisation procedure - either radioactive or non-radioactive probes (oligonucleotide, cDNA and riboprobes)
- Complete analysis - interpretation, high quality microscopy and image analysis, and quantification of hybridisation signal
- FISH - especially tumour diagnostics
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Is your gene expressed at very low levels, but you still need cellular localisation? Orion offers the only current UK-based in situ-(RT)-PCR service to help get round this sensitivity problem.
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For the localisation of the translated protein, using in-house or customer-provided antibodies. A variety of tissue types can be examined including frozen and paraffin-embedded material.
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For co-localisation of nucleic acids and proteins. We can either perform in situ hybridisation and immunohistochemistry on serial sections, or can perform both techniques on the same section for the ultimate in co-localisation studies.
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Routine histological examinations, including preparation of wax-embedded tissue (both soft and calcified), cutting of sections, staining and interpretation.A variety of samples and stains are available for these procedures.
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With many years of experience, our pathologists, histologists and molecular biologists can trouble-shoot even the most difficult of problems.
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Assistance with primer design for PCR and probe design and generation for in situ hybridisation. We can also help to optimise a protocol for use in your own laboratory.
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We hold a bank of normal and diseased tissues. Samples not in stock can also be sourced. In addition to our standard arrays, we provide customised arrays and also can produce tissue arrays from customer-provided material.
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